Dab-Sniper Kem Chromasir HPLC UPLC kem tshem tawm dab peaks

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Ghost-Sniper kem yog cov cuab yeej muaj zog los tshem tawm dab peaks uas tsim thaum lub sij hawm txheej txheem ntawm chromatographic sib cais, tshwj xeeb tshaj yog nyob rau hauv gradient hom. Dab peaks yuav ua rau muaj teeb meem ntau yog tias dab peaks overlap lub ncov ntawm kev txaus siab. Nrog Chromasir dab-sniper kem, tag nrho cov kev cov nyom los ntawm dab peaks tuaj yeem daws tau thiab qhov kev sim noj cov nqi yuav qis dua.

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Khoom cim npe

Dab-sniper kem yog tsim tshwj xeeb los tshem tawm dab peaks. Dab peaks yog tsis paub keeb kwm nyob rau hauv ib tug chromatogram, feem ntau pom thaum lub sij hawm tus txheej txheem ntawm chromatographic sib cais nyob rau hauv gradient elution los yog ntev-lub sij hawm ua hauj lwm. Qhov tshwm sim ntawm dab peaks muaj kev cuam tshuam tob rau cov kws tshuaj ntsuam kev sim. Ghost-sniper kem tshwm sim los daws cov teeb meem thiab ua kom cov txheej txheem sim ua kom yooj yim dua thiab ua haujlwm tau zoo. Cov kab no tuaj yeem siv rau hauv qhov xwm txheej hnyav thiab qhia tau tias muaj txiaj ntsig zoo. Nws yog ib txoj hauv kev zoo los tshem tawm kev cuam tshuam los ntawm dab peaks ntawm kev txheeb xyuas cov txheej txheem thiab kev tshuaj xyuas cov tshuaj lom neeg.

Parameters

Tshooj No.	Dimension	Ntim	Daim ntawv thov
MC5046091 p	50 × 4.6 hli	ib 800ul	HPLC
MC3546092 p	35 × 4.6mm	ua 580ul	HPLC
MC5021093 p	50 × 2.1 hli	ib 170ul	UPLC
MC3040096 p	30 × 4.0 hli	ua 380ul	HPLC Tsawg kab ntim ntim

Kev teeb tsa

Daim ntawv thov thiab cov txiaj ntsig

Cov kev ceev faj

1. Yog hais tias lub batch tsom xam yog teem nyob rau hauv lub HPLC system, nws yog pom zoo kom ntxiv nyiaj tshuav lub sij hawm li ntawm 5 min - 10 min nyob rau hauv koj chromatographic tej yam kev mob, rau lub zog ntawm lub ntim ntawm dab-sniper kem.
2. Rau cov kab tshiab, yaug lawv nrog 100% acetonitrile ntawm tus nqi ntws ntawm 0.5ml / min rau 4 teev ua ntej siv.
3. Ion-pair reagents nyob rau hauv lub sij hawm mobile, tej zaum yuav absorbed los ntawm dab-sniper kem, uas yuav cuam tshuam lub sij hawm tuav thiab lub ncov zoo ntawm koj lub hom phiaj. Thov siv ceev faj nyob rau hauv xws li theem mobile.
4. Cov kab ke lub neej nyob ntawm qhov kev soj ntsuam, xws li lub sijhawm txawb. cov kuab tshuaj purity, thiab cov kab mob ntawm cov khoom siv. Thov hloov tus dab-sniper kab tsis tu ncua kom ntseeg tau tias kev ua tau zoo.
5. Txhawm rau hloov dab-sniper kem yog qhia yog tias kev ntes cov nyhuv tshwm sim los yog xav tau tsis tuaj yeem txaus siab.
6. Raws li kev ua kom huv si rau cov kua chromatograph, dab-sniper kem tuaj yeem lim cov khoom thiab tshem tawm cov pa phem ua ntej txhaj tshuaj. Sab dab dab-sniper tseem muab cov cuab yeej thiab chromatographic kem nrog kev tiv thaiv zoo dua, thiab ua kom tiav chromatogram.
7. Yog hais tias lub xov tooj ntawm tes muaj tsis muaj ntsev, ua ntej thiab tom qab siv, xa mus rau flush nrog 10% organic theem tov (10% methanol los yog acetonitrile), kom tsis txhob buffer ntsev tawm thiab thaiv kab.
8. Thov nco ntsoov tias tsis yog txhua tus dab peaks tuaj yeem ntes tau los ntawm Ghost-Sniper kab.
9. Yog tias cov kab ntawv tawm tsis siv sijhawm ntev, nws raug nquahu kom khaws cia rau hauv cov organic aqueous tov (70% methanol lossis acetonitrile). Thiab thov yaug nws nrog 100% acetonitrile ntawm tus nqi ntws ntawm 0.5 ml / min rau 1 teev ua ntej siv.