Lub ncov meej, ntse ntse yog qhov tseem ceeb rau cov txiaj ntsig tau tseeb hauv Kev Tshawb Fawb Ua Kua Dej Chromatography (HPLC). Txawm li cas los xij, kev ua tiav qhov zoo tshaj plaws ncov tuaj yeem nyuaj, thiab ntau yam tuaj yeem ua rau cov txiaj ntsig tsis zoo. Peak zoo nyob rau hauv HPLC tuaj yeem tshwm sim los ntawm ntau yam teeb meem xws li kab mob sib kis, hnyav tsis sib haum, ntim tuag, thiab kev coj ua tsis zoo. Nkag siab txog cov laj thawj no thiab yuav daws lawv li cas yog qhov tseem ceeb rau kev tswj xyuas qhov tseeb thiab txhim khu kev qha chromatographic.
Qhov cuam tshuam ntawm kab kab mob ntawm Peak Shape
Ib qho ntawm cov laj thawj tseem ceeb ntawm cov duab tsis zoo hauv HPLC yog kab kab mob. Sij hawm dhau mus, cov kab mob kis tau los ntawm cov qauv lossis cov kuab tshuaj tuaj yeem sib sau ua ke hauv kem, ua rau muaj kev sib cais tsis zoo thiab tsis sib haum xeeb. Qhov kev sib kis no tuaj yeem ua rau tailing lossis fronting peaks, ob qho tib si tuaj yeem cuam tshuam rau qhov zoo ntawm koj qhov kev soj ntsuam.
Txhawm rau kom tsis txhob muaj kab mob kab mob, kev tu kom zoo thiab khaws cov kab ke yog qhov tseem ceeb. Ua raws li cov chaw tsim khoom pom zoo rau kev tu cov txheej txheem, thiab siv cov kuab tshuaj purity siab thiab cov qauv npaj kom txo tau cov kab mob. Yog tias tsis muaj kab mob tshwm sim, nws yuav tsum tau hloov cov kab ke.
Solvent Mismatching thiab nws cov teebmeem ntawm Peak Quality
Lwm qhov ua rau muaj qhov tsis zoo ntawm qhov siab tshaj plaws yog qhov tsis sib haum xeeb ntawm cov qauv hnyav thiab cov txheej txheem mobile. Yog tias cov kuab tshuaj tsis sib haum, nws tuaj yeem ua rau cov qauv tsis zoo thiab kev sib cais tsis zoo, ua rau qhov dav dav lossis skewed peaks.
Yuav kom daws tau qhov teeb meem no, nco ntsoov xyuas kom meej tias koj tus qauv hnyav yog sib xws nrog lub sijhawm txawb. Qhov no tuaj yeem ua tiav los ntawm kev siv cov kuab tshuaj nrog cov polarities zoo sib xws lossis los ntawm kev diluting cov qauv kom zoo. Nws kuj yog ib qho kev xyaum zoo los siv cov kuab tshuaj tshiab los tiv thaiv kev tsim ntawm cov precipitates uas yuav cuam tshuam rau kev tsom xam.
Tuag Volume Teeb meem thiab lawv cov kev daws teeb meem
Lub ntim tuag yog hais txog cov cheeb tsam hauv lub cev, xws li lub tshuab txhaj tshuaj lossis lub raj xa dej, qhov twg cov qauv lossis theem ntawm lub xov tooj ntawm tes stagnates. Qhov no tuaj yeem ua rau muaj teeb meem zoo li qhov siab tshaj plaws lossis cov duab tsis sib haum, vim tias cov qauv tsis ntws los ntawm lub system. Cov ntim tuag feem ntau yog tshwm sim los ntawm kev teeb tsa tsis raug lossis siv cov khoom siv uas tsis tsim los rau HPLC daim ntawv thov.
Txhawm rau daws teeb meem ntim tuag, tsis tu ncua xyuas koj lub kaw lus rau txhua qhov chaw uas tus qauv yuav stagnate. Xyuas kom tseeb tias koj cov kev sib txuas yog nruj, cov tubing yog qhov loj, thiab tsis muaj kinks lossis xau. Kev txo qis qhov ntim tuag tuaj yeem txhim kho lub ncov zoo thiab kev daws teeb meem.
Lub luag hauj lwm ntawm cov qauv tuav thiab txhaj tshuaj
Kev coj tus cwj pwm kom zoo yog qhov tseem ceeb heev kom tau txais cov txiaj ntsig raug thiab rov tsim dua tshiab. Ib qho ntawm qhov tsis pom zoo tshaj plaws ntawm cov duab tsis zoo yog qhov siv tsis raug ntawm cov cuab yeej txhaj tshuaj, xws li koob txhaj tshuaj, koob, thiab cov qauv vials. Ib lub syringe qias neeg los yog puas tsuaj tuaj yeem qhia cov kab mob los yog ua rau cov tshuaj tsis sib haum, ua rau cov duab tsis zoo.
Xyuas kom tseeb tias koj ib txwm siv cov koob txhaj tshuaj zoo thiab cov koob txhaj tshuaj huv si, thiab tsis txhob overloading cov qauv vial. Tsis tas li ntawd, siv hom vial kom raug tuaj yeem pab tiv thaiv kom tsis txhob muaj kab mob thiab tswj xyuas qhov sib xws. Tsis tu ncua tshuaj xyuas thiab hloov cov khoom uas puas lawm los yog puas lawm kom ntseeg tau tias kev ua tau zoo.
Yuav Ua Li Cas Txhim Kho Koj Lub HPLC rau Qhov Zoo Tshaj Plaws Zoo
Kev tiv thaiv tsis zoo ncov hauv HPLC pib nrog kev saib xyuas kom zoo. Kev tu tsis tu ncua, ua tib zoo xaiv cov kuab tshuaj, thiab kev coj ua qauv zoo yog qhov tseem ceeb los ua kom muaj kev ua haujlwm zoo chromatographic. Ua raws li cov kauj ruam no los tswj koj qhov system:
Tsis tu ncua ntxuav thiab hloov koj kem raws li cov chaw tsim khoom cov lus qhia.
Siv cov kuab tshuaj purity nkaus xwb thiab npaj koj cov qauv nrog kev saib xyuas kom tsis txhob muaj kab mob.
Txo cov ntim tuag los ntawm kev tshuaj xyuas thiab tswj xyuas koj cov HPLC cov kab ke.
Xyuas kom tus qauv tuav kom zoo nrog cov cuab yeej siv tshuaj zoo huv si thiab cov vials.
Xaus: Ua tiav qhov sib xws, Ntse Peaks nrog kev saib xyuas kom raug
Peak zoo nyob rau hauv HPLC tuaj yeem yog qhov teeb meem ntxhov siab, tab sis los ntawm kev nkag siab txog cov laj thawj thiab ua raws li ob peb kauj ruam yooj yim, koj tuaj yeem txhim kho koj cov txiaj ntsig zoo. Kev kuaj xyuas lub cev tsis tu ncua, kev npaj cov qauv tsim nyog, thiab siv cov khoom siv zoo yog qhov tseem ceeb rau kev tswj xyuas qhov zoo tshaj plaws thiab kev ua haujlwm ntawm chromatographic.
Txhawm rau kom lub neej ntev thiab raug ntawm koj lub HPLC system, nws yog ib qho tseem ceeb uas yuav tsum tau ceev faj thiab ua haujlwm zoo hauv kev saib xyuas. Yog tias koj tab tom ntsib teeb meem nrog lub ncov zoo lossis xav tau kev pab hauv kev txhim kho koj lub HPLC system, hu rauChromasirhnub no rau cov kws tshaj lij cov lus qhia thiab cov kev daws teeb meem raws li koj xav tau.
Lub sij hawm xa tuaj: Plaub Hlis-28-2025