Chromatography yog cov txheej txheem tseem ceeb hauv kev tshawb fawb niaj hnub no, tab sis qhov tshwm sim ntawmdab peakshauv chromatograms tuaj yeem tsim teeb meem loj rau cov kws tshuaj ntsuam. Cov peaks npaj txhij txog no, feem ntau tshwm sim thaum lub sij hawm chromatographic sib cais, tshwj xeeb tshaj yog nyob rau hauv gradient hom, cuam tshuam kev soj ntsuam kom muaj nuj nqis thiab nkim sij hawm zoo. Nkag siab qhov ua rau ntawm dab peaks thiab siv cov kev daws teeb meem tshiab xws li Chromasir'sDab-Sniper Kemtuaj yeem hloov koj cov txheej txheem analytical.
Dab tsi yog Dab Peaks thiab Vim Li Cas Lawv Tseem Ceeb?
Dab peaks yog cov cim qhia tsis pom nyob hauv chromatogram uas cuam tshuam nrog qhov tseeb ntawm cov txiaj ntsig tshuaj ntsuam. Thaum lawv tuaj yeem tshwm sim me me, lawv qhov cuam tshuam tuaj yeem yog qhov tseem ceeb:
1. Kev Sib Tw Quantitative
Thaum dab peaks sib tshooj nrog qhov siab tshaj plaws ntawm kev txaus siab, lawv nyuaj rau qhov tseeb ntawm cov analytes. Qhov no tuaj yeem ua rau cov ntaub ntawv tsis raug txhais lus thiab cov txiaj ntsig tsis txaus ntseeg.
2. Kev daws teeb meem siv sijhawm ntev
Kev txheeb xyuas lub hauv paus ntawm dab peaks feem ntau xav tau kev tshawb nrhiav ntev, cuam tshuam cov kws tshuaj ntsuam xyuas los ntawm cov haujlwm tseem ceeb. Lub sijhawm siv los daws cov teeb meem no tuaj yeem txhim kho cov khoom tsim tau thiab cov txiaj ntsig tshawb fawb.
Dab Ntxwg Nyoog tuaj qhov twg?
Yuav kom tshem tawm cov dab peaks zoo, nws yog ib qho tseem ceeb kom nkag siab txog lawv keeb kwm. Cov peaks uas tsis xav txog no feem ntau tshwm sim los ntawm cov kab mob sib kis hauv:
1.Qhov System Cheebtsam:Cov residues nyob rau hauv lub chromatographic system tuaj yeem ua rau dab peaks.
2.Kem:Cov impurities nyob rau hauv cov khoom ntim los yog hnav los ntawm kev siv yuav ua rau muaj kab mob.
3.Cov qauv:Cov kab mob kis tau qhia txog cov khoom sib txuas uas xav tsis thoob rau hauv chromatogram.
4.Mobile Phase:Cov impurities los ntawm cov kuab tshuaj, tsis muaj ntsev, los yog aqueous/organic theem feem ntau ua rau dab peaks.
5.Ntim:Cov qauv npaj lub raj mis thiab lwm lub thawv tuaj yeem qhia txog cov kab mob seem.
Kev daws teeb meem: Dab-Sniper Kem
Chromasir covDab-Sniper Kemyog qhov kev hloov pauv kev ua si tsim los tawm tsam dab peaks. Kab lus thib ob no muaj cov qauv kev txhim kho thiab cov khoom ntim siab heev, ua rau nws tuaj yeem ntes dab peaks txawm nyob rau hauv huab cua. Nws txoj kev ua tau zoo ua rau nws yog ib qho tseem ceeb rau kev siv cov txheej txheem validation thiab kev soj ntsuam kab.
Cov chaw soj ntsuam siv Ghost-Sniper Kem tau tshaj tawm txog kev txhim kho tseem ceeb hauv chromatogram zoo, txo cov sij hawm daws teeb meem, thiab txhim kho tag nrho cov khoom tsim tau zoo.
Yuav ua li cas kom tau txais txiaj ntsig zoo ntawm Dab-Sniper Kab
Rau kev ua tau zoo, ua raws li cov kev ceev faj thiab kev coj ua zoo tshaj plaws:
1.Lub sij hawm ntsuas qhov ntsuas:
Ntxiv 5–10 feeb ntawm lub sij hawm sib npaug hauv HPLC systems kom haum rau kab ke ntim.
2.Kev teeb tsa thawj zaug:
Tshem cov kab tshiab nrog 100% acetonitrile ntawm tus nqi ntws ntawm 0.5 mL / min rau 4 teev ua ntej siv los xyuas kom meej kev ua haujlwm huv thiab ua haujlwm tau zoo.
3.Kov Ion-Pair Reagents nrog ceev faj:
Ion-pair reagents nyob rau theem mobile tuaj yeem hloov lub sijhawm khaws cia thiab cov duab siab. Siv ceev faj thaum muaj cov tshuaj reagents.
4.Hloov cov kab tsis tu ncua:
Kem lifespan nyob ntawm tej yam xws li mobile theem purity, hnyav tej yam kev mob, thiab khoom huv si. Kev hloov tsis tu ncua ua kom cov txiaj ntsig zoo ib yam.
5.Flush Salt-Containing Mobile Phases:
Siv cov tshuaj 10% organic theem (xws li, methanol los yog acetonitrile) ua ntej thiab tom qab khiav cov ntsev uas muaj cov theem mobile los tiv thaiv kev txhaws.
6.Khaws kom zoo thaum lub sijhawm poob:
Rau kev khaws cia ntev, khaws cov kab hauv 70% organic aqueous tov (methanol lossis acetonitrile). Flush nrog 100% acetonitrile ua ntej rov qab siv los rov ua haujlwm.
7.Saib xyuas Kev Ua Haujlwm:
Hloov kab ntawv yog tias nws qhov kev ntes tau ploj zuj zus lossis yog tias xav tau kev tshuaj ntsuam xyuas ntau dua nws lub peev xwm.
Vim li cas Dab-Sniper Kab yog qhov tseem ceeb rau koj qhov chaw sim
Dab-Sniper Kem yog ntau tshaj li cov cuab yeej daws teeb meem; nws txhim khu kev ua tau zoo tag nrho ntawm koj qhov system chromatographic.
•Eliminates Dab Peaks:Txawm nyob rau hauv cov xwm txheej nyuaj tshaj plaws, kab ke no ntes dab peaks zoo.
•Cov cuab yeej tiv thaiv:Lim tawm cov khoom thiab cov kuab paug organic, tiv thaiv cov cuab yeej chromatographic thiab kab.
•Txhim kho cov ntaub ntawv zoo:Los ntawm kev tshem tawm kev cuam tshuam, kem ua kom huv si, txhim khu kev qha chromatograms.
Maxi Scientific Instruments: Koj tus khub hauv Kev Ntsuas Zoo
At Maxi Scientific Instruments (Suzhou) Co., Ltd., peb muab cov kev daws teeb meem tshiab los txhawb lub chaw soj nstuam thoob ntiaj teb. Peb Ghost-Sniper Kab yog tsim los kom daws tau cov teeb meem ntawm dab peaks, ua kom cov txiaj ntsig raug thiab ua haujlwm zoo dua.
Txhim kho koj Chromatography Hnub no
Tsis txhob cia dab peaks cuam tshuam koj qhov kev tshawb fawb. Kev nqis peev hauv Chromasir's Ghost-Sniper Kab thiab paub qhov txawv ntawm qhov tseeb, kev ua tau zoo, thiab kev thaj yeeb ntawm lub siab. Hu rauMaxi Scientific Instruments (Suzhou) Co., Ltd.niaj hnub no kom paub ntau ntxiv txog seb peb cov kev daws teeb meem tuaj yeem txhawb koj lub chaw kuaj mob li cas. Ua ke, cia peb ua tiav qhov zoo tshaj plaws hauv txhua qhov chromatogram.
Post lub sij hawm: Dec-13-2024