Chromatography yog ib qho kev tshawb fawb tsis tseem ceeb hauv kev tshawb fawb niaj hnub, tab sis rov tshwm sim ntawmDab peaksHauv Chromatograms tuaj yeem xaiv cov nyom rau cov kws tshuaj ntsuam xyuas. Cov ntsiab lus tsis txaus ntseeg, feem ntau tshwm sim thaum lub sijhawm chromatographic sib cais, tshwj xeeb hauv Gradient hom, cuam tshuam rau kev ntsuas ntau ntxiv thiab khib nyiab ntev. Nkag siab txog qhov ua rau ntawm dab Peaks thiab Leverurbulages Cov kev daws tshiab zoo li Chromasir'sTus dab Sniper Kemtuaj yeem hloov koj cov txheej txheem tshuaj ntsuam.
Dab tsi yog dab peaks thiab vim li cas lawv ho ua?
Dab peaks yog unidals unidence nyob rau hauv lub dromatogram uas cuam tshuam nrog kev pom tseeb ntawm kev ntsuas pom tseeb. Thaum lawv yuav tshwm me, lawv cov kev cuam tshuam tuaj yeem yog qhov tseem ceeb:
1. Qhauav cov nyom
Thaum dab Peaks sib tshooj sib tshooj nrog cov ncov siab ntawm kev txaus siab, lawv ua kom pom qhov tseeb ntawm cov kws tshuaj ntsuam xyuas. Qhov no tuaj yeem ua rau cov ntaub ntawv txhais cov ntaub ntawv thiab tsis ntseeg tau.
2. Lub sijhawm siv nrog kev daws teeb meem
Kev txheeb xyuas qhov chaw ntawm cov dab Peaks feem ntau yuav tsum tau tshawb xyuas ntev, dadealing cov kws tshuaj ntsuam xyuas 'tsom los ntawm cov haujlwm tseem ceeb. Lub sij hawm tau siv sijhawm daws cov teeb meem no tuaj yeem txwv tsis pub muaj kev tsim khoom thiab kev tshawb fawb tau txais txiaj ntsig.
Dab tsi yog dab dab tsi los ntawm?
Txhawm rau kom ua tau zoo tshem tawm cov dab pom kev zoo, nws yog qhov tseem ceeb kom nkag siab lawv lub hauv paus pib. Cov Peakset tsis tau npaj txhij dhau los tshwm sim los ntawm cov paug hauv:
1.Cheebtsam:Cov seem nyob rau hauv chromatographic system tuaj yeem pab txhawb rau dab Peaks.
2.Txhua:Impurities nyob rau hauv cov khoom ntim khoom lossis hnav los ntawm kev siv yuav ua rau paug.
3.Kuaj:Cov qauv tsis huv uas qhia txog kev npaj ua teb txawv rau hauv cov chromatogram.
4.Txawb theem:Impurities los ntawm cov kuab tshuaj, cov ntsev tsis sib xws, lossis aqueous / organic kauj xaim feem ntau pab txhawb rau dab.
5.Ntim:Qauv Npaj Los Npaj thiab lwm lub ntim tuaj yeem ua kom paub cov kab mob uas tseem ceeb.
Kev daws teeb meem: The Ghost-Sniper kem
Chromasir'sTus dab Sniper Kemyog kev ua si-hloov cov kev daws tau tsim los tawm tsam dab kis. Cov kab lus thib ob zoo tshaj plaws txhim kho qauv thiab cov ntaub ntawv ntim tau zoo, ua kom nws ntes tau zoo siab dab txawm nyob rau hauv cov xwm txheej huab. Nws cov txiaj ntsig ua nws ib qho cuab yeej tseem ceeb rau txoj kev siv tau thiab kev soj ntsuam kab lig.
Chaw ua hauj sam siv cov dab Sniper sab tau tshaj tawm tseem ceeb hauv Chromatogram zoo, txo cov peev txheej muaj teeb meem, thiab txhim kho cov khoom lag luam tag nrho.
Yuav ua li cas thiaj siab tau txais txiaj ntsig ntawm dab-sniper secections
Rau kev ua tau zoo tshaj plaws, ua raws li cov kev ceevfaj no thiab kev coj ua zoo:
1.Lub sijhawm sib haum xeeb:
Ntxiv 5-10 feeb ntawm kev tshuav sijhawm nyob rau hauv HPLC system kom haum rau kem lub ntim.
2.Pib Teeb:
Flush cov kab ntawv tshiab nrog 100% acetonitrile ntawm tus nqi txaus ntawm 0.5 ml / min rau 4 teev ua ntej siv kev ua haujlwm huv thiab ua haujlwm huv si.
3.Kov ion-khub reagents nrog ceev faj:
Ion-khub reagents hauv cov theem mob tuaj yeem hloov kho lub sijhawm thiab lub ntsej muag ci. Siv kev ceev faj thaum cov tshuaj tiv thaiv no muaj.
4.Hloov cov kab niaj zaus:
Cov nyiaj seem yog ntawm qhov zoo li mobile theem kev coj dawb huv, cov kuab tshuaj huab cua, thiab cov cuab yeej huv. Hloov pauv hloov ua kom ua tau zoo sib xws.
5.Flush ntsev-muaj cov theem no mobile:
Siv 10% cov kev daws teeb meem theem (piv txwv li, tshuaj tua kab mob me lossis acetoniturile) ua ntej thiab tom qab khiav cov theem ntsuas kom tiv thaiv kom tsis txhob muaj kev cuam tshuam.
6.Khw kom zoo thaum lub sijhawm poob qis:
Rau lub sijhawm ntev cia, khaws cov kem hauv 70% organic aqueous daws (methanol lossis acetoniturile). Yaug nrog 100% acetonitrile ua ntej rov ua haujlwm.
7.Saib xyuas kev ua tau zoo:
Hloov cov kab ntawv yog tias nws yuav ntes tau txo qis zuj zus lossis yog tias kev ntsuas kev xav tau tshaj li nws lub peev xwm.
Vim Li Cas Tsom-Sniper Txhua yog qhov tseem ceeb rau koj li kuaj
Tus dab-sniper kem yog ntau tshaj li cov cuab yeej daws teeb; Nws txhim kho qhov kev ua tau zoo tag nrho ntawm koj cov tshuaj chromatographic.
•Tshem tawm cov dab Peaks:Txawm nyob rau hauv cov xwm txheej nyuaj tshaj plaws, cov kab no tau ntes dab pom kev zoo siab.
•Tiv thaiv cov khoom siv:Lim tawm cov khoom siv khov kho thiab cov organic paug, tiv thaiv cov twj paj nruag chratographic thiab kab.
•Txhim kho cov ntaub ntawv zoo:Los ntawm kev tshem tawm kev cuam tshuam, kem tsim tawm huv, ntau txhim khu kev qha cov qau.
Maxi Scientific: Koj tus khub hauv kev tshawb pom kev ua tau zoo
At Maxi Scientific (Suzhou) Co., Ltd., peb muab cov kev daws teeb meem tshiab rau Empower Chaw Haujlwm Thoob Ntiaj Teb Thoob Ntiaj Teb. Peb tus dab Sniper yog qhov ua kom muaj kev sib tw los daws cov kev cov nyom ntawm cov dab ntxim siab, kom muaj txiaj ntsig zoo thiab cov haujlwm smoother.
Txhim kho koj cov chromatography hnub no
Tsis txhob cia dab Peaks cuam tshuam koj cov kev tshawb fawb. Nqes peev hauv Chromasir lub dab-sniper-sniper sacts thiab paub qhov sib txawv hauv qhov tseeb, kev ua tau zoo, thiab kev thaj yeeb ntawm lub siab. Tus neeg yus paubMaxi Scientific (Suzhou) Co., Ltd.Niaj hnub no kom paub ntau ntxiv txog yuav ua li cas peb cov kev daws teeb meem tuaj yeem tsim kho koj qhov kev kuaj mob. Ua ke, cia ua tiav qhov zoo tshaj plaws nyob rau hauv txhua checkomogram.
Lub Sijhawm Post: Dec-13-2024