Khaws koj cov kab chromatography nyob rau hauv qhov kev pom zoo tsis yog tsuas yog kev coj ua zoo xwb - nws yog qhov tseem ceeb rau cov txiaj ntsig raug thiab cov nqi mus sij hawm ntev. Txawm hais tias koj ua haujlwm hauv kev tshuaj xyuas tshuaj, kev nyab xeeb khoom noj, lossis kev sim ib puag ncig, kev kawm yuav ua li cas txuas ntxiv lub neej ntawm koj cov chromatography kab yuav txo qis sijhawm, txhim kho kev tsim tawm, thiab pab tswj kev ua haujlwm zoo ib yam.
Kev khaws cia kom zoo ua rau txhua qhov sib txawv
Ib qho ntawm qhov tsis pom zoo tshaj plaws ntawm kev saib xyuas kab ke yog kev cia kom zoo. Kev cia khoom tsis raug tuaj yeem ua rau muaj kev loj hlob ntawm microbial, hnyav evaporation, thiab kev puas tsuaj tsis tuaj yeem. Ib txwm ua raws li cov lus qhia tsim nyog raws li hom chromatography kab uas koj siv. Piv txwv li, thaum khaws cov kab rov qab-theem rau ncua sij hawm, yaug nrog cov sib tov uas muaj tsawg kawg yog 50% cov kuab tshuaj organic, thiab kaw ob qho tib si kom nruj. Yog tias koj siv buffered mobile theem, tsis txhob cia cov buffer qhuav hauv kem, vim qhov no tuaj yeem ua rau ntsev los nag thiab txhaws.
Tiv thaiv clogging thiab paug
Kev zam kom tsis txhob kis kab mob yog ib txoj hauv kev zoo tshaj plaws los ua kom lub neej ntev. Kev lim dej ntawm cov theem mobile thiab cov qauv yog qhov tseem ceeb. Siv 0.22 µm lossis 0.45 µm lim kom tshem tawm cov khoom ua ntej txhaj tshuaj. Tsis tas li ntawd, tsis tu ncua hloov cov ntsaws ruaj ruaj, syringes, thiab cov qauv vials kom ntseeg tau tias tsis muaj teeb meem txawv teb chaws nkag mus rau hauv lub cev. Rau cov chaw soj nstuam uas ua haujlwm nyuaj lossis qias neeg, kab ke saib xyuas tuaj yeem ua thawj kab ntawm kev tiv thaiv tus qauv cuam tshuam nrog fouling-nqus cov kab mob ua ntej lawv mus txog ntawm kab tshuaj ntsuam.
Routine Flushing thiab ntxuav yog tsis sib tham
Yog tias koj cov kab chromatography siv tsis tu ncua, kev yaug dej yog qhov tseem ceeb. Kev tu ncua sij hawm tshem tawm cov khoom seem uas tuaj yeem ua rau lub suab nrov nrov, dab peaks, lossis poob kev daws teeb meem. Flush lub kem nrog cov kuab tshuaj sib haum nrog lub sijhawm txawb tab sis muaj zog txaus los ntxuav tawm cov khoom khaws cia. Rau cov kab rov qab-theem, sib tov dej, methanol, lossis acetonitrile ua haujlwm zoo. Muab cov sij hawm tu txhua lub lim tiam raws li qhov zaus thiab hom kev tshuaj ntsuam xyuas los tiv thaiv kev tsim thiab ua kom muaj txiaj ntsig zoo.
Siv Pre-kem Lim thiab Guard Columns
Kev txhim kho lub lim dej ua ntej lossis kab thaiv kab ke yog kev nqis peev me me nrog cov nyiaj rov qab loj. Cov Cheebtsam no ntes cov particulates thiab muaj zog khaws cia cov tebchaw ua ntej lawv tuaj yeem nkag mus rau hauv lub ntsiab analytical kem. Lawv tsis tsuas yog txuas lub neej ntawm koj cov chromatography kem tab sis kuj tiv thaiv nws los ntawm kev kub ntxhov tshwm sim los ntawm kev cuam tshuam. Txawm hais tias cov khoom siv no xav tau kev hloov pauv raws sijhawm, lawv pheej yig dua li hloov tag nrho cov kab ke kev tshuaj ntsuam.
Cov lus qhia txog kev tu rau cov neeg siv HPLC
Rau cov neeg siv HPLC, kev saib xyuas lub zog thiab cov nqi ntws tuaj yeem muab cov cim qhia ntxov ntawm kab degradation. Sudden nce nyob rau hauv rov qab siab feem ntau qhia clogging, thaum lub sij hawm drifting tuav yuav qhia ib feem ntawm blockage los yog theem degradation. Kev siv tus nqi txaus txaus thiab zam qhov kev hloov pauv siab yuav tiv thaiv kev ncaj ncees ntawm ob qho tib si ntawm cov kab ntim khoom thiab nws cov theem nyob ruaj khov. Tsis tas li ntawd, tsis txhob nthuav tawm cov kab rau hauv cov kuab tshuaj tsis sib haum lossis pH nyob sab nraum nws qhov kev pom zoo, vim tias cov no tuaj yeem ua rau muaj kev puas tsuaj sai.
Kev xav zaum kawg
Koj cov kab chromatography yog ib qho tseem ceeb ntawm koj qhov kev tshuaj ntsuam xyuas, thiab nrog kev saib xyuas zoo, nws tuaj yeem xa ntau txhiab qhov kev txhaj tshuaj zoo. Los ntawm kev khaws cia kom zoo rau kev tu thiab kev pom zoo, txais yuav kev saib xyuas-thawj lub tswv yim tsis tsuas yog khaws koj cov ntaub ntawv zoo xwb tab sis kuj txo cov nqi hloov.
Nrhiav kom zoo dua koj lub chaw kuaj chromatography workflow? Tshawb nrhiav cov kev daws teeb meem thiab cov kws tshaj lij cov lus qhia ntawmChromasir- qhov twg precision ntsib kev ntseeg tau. Cia peb pab txuas ntxiv lub neej ntawm koj cov khoom siv thiab txhawb koj cov txiaj ntsig.
Post lub sij hawm: Apr-23-2025