Hauv kev ua haujlwm siab ua kua chromatography (HPLC), ob peb yam tseem ceeb heev - lossis kim - raws li kab chromatography. Tab sis koj puas paub tias nrog kev saib xyuas kom raug thiab tuav, koj tuaj yeem txuas ntxiv koj lichromatography kab lifespanthiab txhim kho koj qhov kev ua haujlwm tag nrho?
Phau ntawv qhia no tshawb nrhiav cov lus qhia txog kev saib xyuas thiab cov tswv yim tswv yim uas tuaj yeem pab koj tiv thaiv koj cov peev txheej thiab ua kom muaj txiaj ntsig zoo sib xws hauv lub sijhawm.
Xaiv Txoj Cai Mobile Phase los ntawm Pib
Txoj kev taug mus ntev duachromatography kab lifespanpib nrog kev xaiv cov kuab tshuaj ntse. Qhov tsis ncaj ncees lawm lub xov tooj ntawm tes tuaj yeem ua rau cov khoom ntim khoom tsis zoo, txo cov kev daws teeb meem, lossis tseem ua rau muaj kev puas tsuaj tsis zoo. Nco ntsoov xyuas cov pH, ionic zog, thiab hnyav hom yog sib xws nrog koj cov chemistry tshwj xeeb.
Degassing cov kuab tshuaj thiab lim lawv ua ntej siv kuj yog cov kauj ruam tseem ceeb. Cov kev ceev faj yooj yim no tiv thaiv tsis pub muaj qhov txhaws thiab cov pa npuas tsim, ob qho tib si tuaj yeem cuam tshuam kev ua haujlwm ntawm kab.
Txhim kho koj cov txheej txheem txhaj tshuaj
Dab tsi nkag mus rau hauv kab ntawv tseem ceeb npaum li nws mus txog qhov ntawd. Cov qauv hnyav dhau los lossis cov uas muaj cov khoom tuaj yeem ua kom luv luv ntawm kab ke siv lub neej. Siv cov qauv npaj tau zoo - lim los ntawm 0.22 lossis 0.45 µm cov lim dej - txhawm rau tiv thaiv kev txhaws thiab ua kom siab.
Yog tias koj ua haujlwm nrog cov matrices nyuaj lossis qias neeg, xav txog kev siv cov kem saib xyuas lossis lim ua ntej. Cov khoom siv pheej yig no tuaj yeem cuam tshuam cov kab mob ua ntej lawv mus txog ntawm kab ntawv txheeb xyuas, nthuav dav heevchromatography kab lifespan.
Tsim kom muaj kev tu ncua tsis tu ncua
Ib yam li txhua yam khoom siv precision, koj kem xav tau kev tu ib txwm ua kom muaj kev ua tau zoo tshaj plaws. Ib qho kev xyaum zoo yog yaug cov kab tom qab txhua qhov kev siv nrog cov kuab tshuaj sib xws, tshwj xeeb tshaj yog thaum hloov ntawm cov tshuab tsis siv neeg lossis cov qauv qauv.
Periodic sib sib zog nqus ntxuav nrog cov kuab tshuaj muaj zog tuaj yeem tshem tawm cov khib nyiab thiab cov tshuaj hydrophobic. Nco ntsoov ua raws li kab ke kev tu vaj tse tshwj xeeb thiab tsis txhob siv cov tshuaj phem uas yuav ua rau lub sijhawm nyob ruaj khov.
Khaws Nws Txoj Cai Ntawm Kev Ua Haujlwm
Kev khaws cia kom zoo yog feem ntau tsis saib xyuas, tab sis nws ua lub luag haujlwm tseem ceeb hauv kev khaws cia kojchromatography kab lifespan. Yog tias ib kem yuav tsis siv rau lub sijhawm ntev, nws yuav tsum tau yaug nrog cov kuab tshuaj uas tsim nyog - feem ntau muaj cov organic tivthaiv tiv thaiv kev loj hlob ntawm microbial.
Ib txwm hau ob qhov kawg kom nruj kom tsis txhob ziab tawm los yog muaj kab mob. Rau kev khaws cia ntev, khaws cov kab hauv qhov huv si, tswj qhov kub thiab txias, kom deb ntawm lub teeb ncaj qha thiab cua sov.
Saib xyuas kab kev ua haujlwm tsis tu ncua
Khaws lub log ntawm lub siab rov qab, lub sijhawm khaws cia, thiab lub ncov zoo tuaj yeem pab koj txheeb xyuas cov cim ntxov ntawm kab degradation. Kev hloov tam sim ntawd hauv cov kev ntsuas no yuav qhia tau tias muaj kab mob sib kis, voids, lossis frit clogging.
Los ntawm kev ntes cov teeb meem no ntxov, koj tuaj yeem ua qhov kho kom raug-xws li ntxuav lossis hloov kab thaiv-ua ntej lawv cuam tshuam rau koj cov txiaj ntsig kev soj ntsuam tas li.
Kev xav zaum kawg
Extending kojchromatography kab lifespantsis yog hais txog kev txuag nyiaj xwb-nws yog hais txog kev tswj cov ntaub ntawv ncaj ncees, txo qis qis qis, thiab txhim kho cov khoom siv hauv chav kuaj. Nrog txoj kev tiv thaiv kev saib xyuas zoo, koj tuaj yeem tiv thaiv ib qho ntawm koj cov khoom muaj nqis tshaj plaws hauv chav kuaj thiab xyuas kom muaj txiaj ntsig zoo dua hauv txhua qhov kev khiav.
Xav tau cov kws tshaj lij cov lus qhia txog kev coj ua chromatographic lossis xaiv cov khoom?Hu rauChromasirhnub no-peb nyob ntawm no los txhawb koj lub chaw kuaj kev ua tiav nrog kev pom zoo thiab kev daws teeb meem tus kheej.
Lub sij hawm xa tuaj: Plaub Hlis-11-2025