Hauv kev tshawb nrhiav lub chaw soj ntsuam,Kev ua haujlwm siab ua kua chromatography (HPLC)Puas yog ib qho txheej txheem tseem ceeb rau kev sib cais, paub txog, thiab ntau yam kev sib txuas. Txawm li cas los xij, ua tiav cov txiaj ntsig zoo ib yam thiab ntseeg tau xav tau ntau tshaj li cov khoom siv txoj cai-nws yuav tsum taukev ua kom ua kom zooCov. Kab lus no tshawb nrhiav koj yuav ua li cas txhim kho kojHepl Kev Ntsuam XyuasYuav kom ua hauj lwm zoo tshaj plaws, txo qis downtime, thiab txhim kho qhov raug.
Cov kev cov nyom feem ntau hauv HPLC Kev Tshawb Xyuas thiab yuav daws tau lawv li cas
Thaum HPLC yog lub cuab yeej analytical haib, nws tsis yog yam tsis muaj kev cov nyom. Cov teeb meem xws liKev daws teeb meem tsis zoo, suab nrov, thiab tsis sib xwstuaj yeem cuam tshuam cov ntsuas kev ua haujlwm. Ntawm no yog yuav ua li cas hais daws cov teeb meem tshwm sim no:
1. Cov kev daws teeb meem tsis zoo
Ib qho teeb meem feem ntau hauv HPLC yog kev sib cais tsis zoo ntawm peaks, feem ntau vim yogCov kab ntawv xaiv tsis raug lossis suboptimal Flow tus nqiCov. Txhawm rau txhim kho daws teeb meem:
• Xaiv ibChromatographic kemnrog qhov tsim nyogTheem nyob ruaj khov ua haujlwm thiab cov particle lojrau koj cov kws tshuaj ntsuam.
• Khotxaus tus nqi thiab gradient mobTxhawm rau txhim kho qhov tseem ceeb tshaj plaws thiab sib cais.
• sivTswj Qhov Kubkom ruaj khov rau lub sijhawm thiab txhim kho qhov reppucibility.
2. Baseline drift lossis suab nrov
Cov suab nrov hauv paus tuaj yeem cuam tshuam nrog cov ntaub ntawv ntsuas pa kom zoo thiab kho cov ntaub ntawv raug. Qhov teeb meem no feem ntau tshwm sim los ntawm:
•Kev hloov pauv kub- Tswj ib puag ncig ruaj khov thiab siv cov cub kem yog tsim nyog.
•Kev tsis huv lub sijhawm pham- Siv cov kuab tshuaj coob heev thiab lim koj theem mobile ua ntej siv.
•Ntsuas cov khoom tsis huv- Tsis tu ncua huv si thiab khaws lub ntsuas, twj tso kua mis, thiab tubing mus txo cov suab nrov tom qab.
3. Kev sib xyaw ua ke
Kev sib xyaw ua ke cuam tshuam rau kev ntseeg tau ntawm kev muaj nuj nqis. Los daws qhov no:
• Ua kom covHPLC Kem yog qhov tseem ceebua ntej siv.
• tswj hwmTus nqi ntws ruaj khovthiab tiv thaiv kev hloov siab siab.
• Txhim khosoftware chaw rau kev ua ke, ua kom pom kev zoo ib yam thiab cov txiaj ntsig rov ua dua.
Xaiv lub HPLC yog HPLC
Xaiv cov ntawv HPLC yogQhov tseem ceeb rau kev ua tiav kev sib cais zoo tshaj plawsCov. Xav txog cov xwm txheej no thaum xaiv ib kem:
•Sab Ntev: Cov kab ntev muab kev sib cais zoo dua tab sis nce sijhawm ntsuas. Xaiv qhov ntev uas sib npaug daws teeb meem thiab nrawm.
•Kab intaib: Cov kab nqaim muaj cov kev daws teeb meem siab dua tab sis xav tau siab ntau dua. Xyuas kom haum nrog koj lub HPLC system.
•Theem nyob ruaj khov: Xaiv ib theem nrog haum rau kev siv tshuaj rau koj cov kws tshuaj ntsuam (piv txwv li, c18 rau cov tebchaw tsis yog, phenyl rau cov duab uas muaj ntxhiab).
Ua kom zoo rau pheeb kis mob thiab ntws
Cov mobile theem yog tus yuam sij rau kev tsom xam hplc. Ntawm no yog yuav ua li cas zoo rau nws:
•Kho cov kuab cua sib xyaw: Nplua-kaw lubHnyav Piv Txwvtxhawm rau txhim kho kev sib cais. Kev sivGradient elientrau cov qauv nyuaj.
•Tswj Ph theem: Kom covTxawb theem phyog sib xws nrog ob qho qauv thiab kem.
•Ua kom zoo tus nqi Flat: Cov nqi nce siab txo qis kev soj ntsuam lub sijhawm tab sis tej zaum yuav muaj kev cuam tshuam daws teeb meem. Sib npaug ceev thiab ua tau zoo raws li koj txoj kev.
Saib Xyuas thiab Tiv Thaiv Kev Tiv Thaiv
Tsim Kho Kom Zookev ua tau zoo ib yam thiab ncua sij hawm ntsuas lub neejCov. Ua raws cov kev coj ua zoo tshaj plaws:
•Kev Tu Kom Huv Si: Nquag ntxuav lubInjector, kem, thiab nteslos tiv thaiv kev sib kis.
•Hloov pauv cov neeg siv khoom: HloovNtsaws, lim dej, thiab tubingraws li xav tau los tiv thaiv kom tsis txhob muaj xau thiab siab siab hloov.
•Kho kom haum rau lub system: Tsis tu ncua ntsuas hluav taws xob thiab lwm yam tseem ceeb kom ntseeg tau tias tau txais txiaj ntsig.
Tag
Ua kom zoo HPLC tsom xam yog qhov tseem ceeb rau kev txhim kho kev ua tau zoo thiab ua kom muaj txiaj ntsig zoo. Los ntawm kev daws cov teeb meem uas nquag muaj xws liKev daws teeb meem tsis zoo, suab nrov, thiab kev koom ua ke tsis sib xyaw, thiab xaiv txoj caiTxhua thiab cov theem mob tes, koj tuaj yeem txhim kho koj qhov kev ntsuas pom zoo. Ib txwmKev saib xyuas thiab ceev faj txoj kev optimizationYuav ua kom koj lub HPLC system khiav ntawm lub ncov efficiency, txo qis downtime thiab ua kom muaj tseeb, rov ua tau zoo.
Rau cov kws qhia txog cov kws tshaj lij ntawmHPLC Optimization, huTus roj lws suav-Peb tshwj xeeb hauv kev muabCustomized chromatography SolutionsTxhawm rau pab koj lub chaw kuaj kom ua tiav cov qauv siab tshaj plaws ntawm kev ua tau zoo.
Lub sij hawm ncua: Mar-27-2025